RPTOR Antibody

Product Discontinued

RPTOR Antibody Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from the central region of human Raptor. The immunogen is located within amino acids 910 - 960 of Raptor.
• Specificity: Raptor has multiple isoforms that may also be recognized by antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPTOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Simple Western
  • Western Blot 1:100-1:2000
• Application Notes: Raptor antibody can be used for the detection of Raptor by Western blot at 2 mg/ml. Raptor has multiple isoforms that may also be recognized by antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RPTOR Antibody

• KIAA1303
• KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
• KOG1
• Mip1
• p150 target of rapamycin (TOR)-scaffold protein
• Raptor
• regulatory associated protein of MTOR, complex 1
• regulatory-associated protein of mTOR
• RPTOR

Background

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPTOR
• Uniprot:
  • Q8N122(Human)