RPSA Antibody (MLuC5)

Western Blot: RPSA Antibody (MLuC5) [NB200-631] - Analysis of 25 ug of HepG2 (lane 1) and Hela (lane 2) cell lysates.

Immunocytochemistry/Immunofluorescence: RPSA Antibody (MLuC5) [NB200-631] - Analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: RPSA Antibody (MLuC5) [NB200-631] - Human breast carcinoma stained with Laminin Receptor antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.

Flow Cytometry: RPSA Antibody (MLuC5) [NB200-631] - Analysis of Laminin Receptor in MCF-7 cells (green) compared to an isotype control (blue).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: MLuC5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RPSA Antibody (MLuC5) Summary

• Description: Novus Biologicals Mouse RPSA Antibody (MLuC5) (NB200-631) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Small cell lung carcinoma N592 live cells
• Localization: Cell membrane with some cytoplasmic.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPSA
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 4 - 8 ug/mL
  • Western Blot 1:10 - 1:200

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21320497).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for RPSA Antibody (MLuC5)

• 37 kDa laminin receptor precursor
• 37/67 kDa laminin receptor
• 37LRPNEM/1CHD4
• 67 kDa laminin receptor
• 67kD, ribosomal protein SA
• 67LR
• Colon carcinoma laminin-binding protein
• LAMBR37 kDa laminin receptor
• laminin binding protein
• laminin receptor 1 (67kD, ribosomal protein SA)
• Laminin receptor 1
• Laminin-binding protein precursor p40
• LAMR 1
• lamR
• LAMR140S ribosomal protein SA
• LBP
• LBP/p40
• LRP
• LRP/LR
• Multidrug resistance-associated protein MGr1-Ag
• p40
• ribosomal protein SA

Background

Laminin, one of the major glycoproteins of basement membrane, displays multiple biological activities which are mediated through its interaction with specific cell membrane receptors. Overexpression of laminin receptor is reportedly an independent prognostic factor in breast cancer and correlates with the dissemination of the tumor cells in bone marrow. Laminin receptor appears to play an important role in tumor invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPSA
• Entrez:
  • 3921(Human)
• Uniprot:
  • P08865(Human)