RPL29 Antibody

Western Blot: RPL29 Antibody [NBP3-12600] - Lane A: PC-3 Whole Cell LysateLane B: U87-MG Whole Cell LysateLane C: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:22 kDa

Immunocytochemistry/Immunofluorescence: RPL29 Antibody [NBP3-12600] - Staining of RPL29 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RPL29 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP3-12600] - Staining of human RPL29 in human liver with rabbit polyclonal antibody at 1:10000 dilution.

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP3-12600] - Staining of human RPL29 in human brain with rabbit polyclonal antibody at 1:10000 dilution.

Immunoprecipitation: RPL29 Antibody [NBP3-12600] - Lane A:0.5 mg PC-3 Whole Cell Lysate4 uL anti-RPL29 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RPL29 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size :22 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RPL29 Antibody Summary

• Immunogen: E. coli-derived Human RPL29 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL29
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000-1:20000
  • Immunoprecipitation 1-5ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.0
• Preservative: 0.03% Proclin 300
• Purity: Protein A purified

Notes

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Alternate Names for RPL29 Antibody

• 60S ribosomal protein L29
• Cell surface heparin-binding protein HIP
• heparin/heparan sulfate-binding protein
• heparin/heparan sulfate-interacting protein
• HIPHUMRPL29
• HP/HS-interacting protein
• MGC88589
• ribosomal protein L29

Background

RPL29, also known as 60S ribosomal protein L29, is a 18kDa 159 amino acid protein and belongs to the L29E family of ribosomal proteins. The primary function of RPL29 is to encode a cytoplasmic ribosomal protein. Current research is being conducted on RPL29 and its relationship with the diseases colon cancer, thyroid carcinoma, lung carcinoma, colorectal cancer, thyroiditis, carcinoma, breast cancer and malaria. RPL29 has been linked to the ossification, localization, pathogenesis, cell adhesion and cell proliferation pathways where it interacts with UBC, SYBU, CHD3, RPL24 and RPL6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPL29