RPL11 Antibody

Western Blot: RPL11 Antibody [NBP2-20210] - Binding to six Anopheles gambiae pupal lysis samples at 21.8 kDa, and the murine control at 20.6 kDa. Image from a verified customer review.

Immunohistochemistry-Paraffin: RPL11 Antibody [NBP2-20210] - RPL11 antibody detects RPL11 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. RPL11 antibody dilution: 1:500.

Western Blot: RPL11 Antibody [NBP2-20210] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: RPL11 Antibody [NBP2-20210] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry Whole Mount: RPL11 Antibody [NBP2-20210] -: RPL11 Antibody [NBP2-20210] - RPL11 antibody detects Rpl11 protein on whole-mount zebrafish embryos by immunohistochemical analysis.? Sample: Paraformaldehyde-fixed zebrafish embryos. RPL11 antibody (NBP2-20210) dilution: 1:200.

• Reactivity: Hu, Mu, I, Ze, Rt, Po, Bv, Ca, Ch, Xp, Ze
• Applications: WB, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RPL11 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPL11. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Porcine (99%), Bovine (100%), Zebrafish (98%), Chicken (98%), Canine (100%), Xenopus (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:500 - 1:3000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%), Drosophila (85%). Anopheles gambie (insect) reactivity reported by a customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RPL11 Antibody

• cell growth-inhibiting protein 34,60S ribosomal protein L11
• CLL-associated antigen KW-12
• DBA7
• GIG34
• ribosomal protein L11

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternative splice variants encoding different isoforms may exist, but they have not been fully characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for RPL11 Antibody (NBP2-20210) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Anopheles gambiae and Anopheles gambie.

reviewed by: Andrea Smidler WB Anopheles gambiae and Anopheles gambie 07/24/2019

Summary

• Application: Western Blot
• Sample Tested: Pupae cell lysis extracts,Pupal lysate
• Species: Anopheles gambiae and Anopheles gambie
• Lot: 40744

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: RPL11
• Uniprot:
  • P62913(Human)