RPA2 Antibody

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Western Blot: RPA2 Antibody [NB100-332] - Detection of Human and Mouse RPA32 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity ...read more
Immunohistochemistry: RPA2 Antibody [NB100-332] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Western Blot: RPA2 Antibody [NB100-332] - Whole cell lysate from 293T cells.
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - FFPE section of human ovarian tumor. Affinity purified rabbit anti-RPA32 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - FFPE section of mouse teratoma. Affinity purified rabbit anti-RPA32 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - FFPE section of human breast tumor. Affinity purified rabbit anti-RPA32 used at a dilution of 1:100.
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM3 (NB100-249) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM4 (NB100-250) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1(NB100-287) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RPA2 Antibody [NB100-332] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
   

Biological Strategies

     

Order Details

RPA2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
RPA2 Lysate (NBL1-15489)
Publications
Read Publications using NB100-332.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPA2 Antibody

  • REPA2
  • Replication factor A protein 2
  • replication protein A 32 kDa subunit
  • Replication protein A 34 kDa subunit
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
  • RF-A protein 2
  • RP-A p32
  • RP-A p34
  • RPA2
  • RPA32
  • RPA34

Background

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA-32, is phosphorylated during the S-phase of the cell cycle, in response to DNA damage and during apoptotsis of Jurkat T- lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RPA2 Antibody (NB100-332) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RPA2 Antibody (NB100-332)

Discover related pathways, diseases and genes to RPA2 Antibody (NB100-332). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RPA2 Antibody (NB100-332)

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Pathways for RPA2 Antibody (NB100-332)

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PTMs for RPA2 Antibody (NB100-332)

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Research Areas for RPA2 Antibody (NB100-332)

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Blogs on RPA2.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RPA2
Entrez
Uniprot