RP105/CD180 Antibody

Western Blot: RP105/CD180 Antibody [NBP2-98285] - Lane A: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:84 kDaObserved band size:70 kDa

Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP2-98285] - Staining of mouse RP105/CD180 in mouse spleen with rabbit polyclonal antibody (1:10000).

Product Discontinued

RP105/CD180 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Mouse RP105/CD180 (Accession#: NP_032559.2; Met1-Ser626)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD180
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunohistochemistry 1:5000-1:20000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for RP105/CD180 Antibody

• CD180 antigenMGC126233
• CD180 molecule
• CD180
• Ly64
• LY64MGC126234
• Ly78
• Lymphocyte antigen 64
• lymphocyte antigen-64, radioprotective, 105kDa
• Radioprotective 105 kDa protein
• RP105
• RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD180