ROR gamma/RORC/NR1F3 Antibody (695935)

Western blot shows lysates of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROR gamma/RORC/NR1F3 Monoclonal Antibody (Catalog # MAB61091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma/RORC/NR1F3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

ROR gamma/RORC/NR1F3 Antibody (695935) Summary

• Immunogen: E. coli-derived recombinant human ROR gamma
  Met1-Gln121
  Accession # P51449
• Specificity: Detects human ROR gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ROR1, rhROR2, rhROR gamma t, or rhROR alpha is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RORC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR gamma/RORC/NR1F3 Antibody (695935)

• MGC129539
• NR1F3
• Nuclear receptor ROR gamma
• Nuclear receptor ROR-gamma
• Nuclear receptor RZR gamma
• Nuclear receptor RZR-gamma
• Nuclear receptor subfamily 1 group F member 3
• RAR-Related Orphan Nuclear Receptor Variant 2
• RAR-related orphan receptor C
• RAR-related orphan receptor gamma
• retinoic acid-binding receptor gamma
• retinoid-related orphan receptor gamma
• Retinoid-related orphan receptor-gamma
• ROR gamma
• RORC
• RORG
• RORGMGC129539
• RZRG
• RZRGRZR-GAMMA
• TOR

Background

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ROR gamma/RORC/NR1F3 Antibody (MAB61091). (Showing 1 - 4 of 4 FAQ).

1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
   • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
   • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
3. Does this ROR gamma/RORC/NR1F3 antibody come in lyophilized form?
   • Yes, we do carry this RORC antibody in lyophilized format.
4. What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?
   • A sequence from Met1 - Gln121.

Bioinformatics

• Gene Symbol: RORC
• Uniprot:
  • P51449()