ROMO1 Antibody (OTI2C12)

Western Blot: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROMO1.

Immunocytochemistry/Immunofluorescence: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of HeLa cells.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Adenocarcinoma of Human breast tissue.

Western Blot: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of extracts (10ug) from 7 different cell lines.

Immunocytochemistry/Immunofluorescence: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ROMO1.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Human breast tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Human prostate tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Human pancreas tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Human Kidney tissue.

Immunohistochemistry: ROMO1 Antibody (OTI2C12) [NBP2-45607] - Analysis of Carcinoma of Human thyroid tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI2C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ROMO1 Antibody (OTI2C12) Summary

• Immunogen: Full length human recombinant protein of human ROMO1(NP_542786) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROMO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:31945745). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ROMO1 Antibody (OTI2C12)

• reactive oxygen species modulator 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROMO1 Antibody (NBP2-45607)(2)

Publications using NBP2-45607

• Liu B, Tian y, Li y et al. ACEA Attenuates Oxidative Stress by Promoting Mitophagy via CB1R/Nrf1/PINK1 Pathway after Subarachnoid Hemorrhage in Rats Oxidative Medicine and Cellular Longevity 2022-02-24 [PMID: 35251464] (WB, Rat)
• Sun G, Cao Y, Qian C et Al. Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages Aging (Albany NY) 2020-01-16 [PMID: 31945745] (WB, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: ROMO1