ROCK2 Antibody

Images

 
Western Blot: ROCK2 Antibody [NBP2-20199] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE gel, diluted at 1:500.
Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (50 ug of whole cell lysate) A: Rat brain, 5% SDS PAGE gel, diluted at 1:1000.
Genetic Strategies: Knockdown Validated: ROCK2 Antibody [NBP2-20199] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

ROCK2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.
Localization
Cytoplasm (By similarity); Cell membrane; Peripheral membrane protein (By similarity)
Predicted Species
Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROCK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ROCK2 Antibody

  • coiled-coil-containing protein kinase 2
  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA0619
  • p164 ROCK-2
  • Rho kinase 2
  • rho-associated protein kinase 2
  • Rho-associated, coiled-coil containing protein kinase 2
  • ROCK2
  • ROCK-II
  • ROKalpha

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ROCK2
Uniprot