ROCK2 Antibody

Images

 
Western Blot: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL
Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue with ROCK2 antibody at 5 ug/mL.
Immunofluorescence: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue with ROCK2 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ROCK2 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2. The immunogen is located within amino acids 920 - 970 of ROCK2.
Specificity
Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROCK2
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunofluorescence 20 ug/ml
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ROCK2 Knockout HeLa Cell Lysate
Control Peptide
ROCK2 Peptide (NBP1-76570PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for ROCK2 Antibody

  • coiled-coil-containing protein kinase 2
  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA0619
  • p164 ROCK-2
  • Rho kinase 2
  • rho-associated protein kinase 2
  • Rho-associated, coiled-coil containing protein kinase 2
  • ROCK2
  • ROCK-II
  • ROKalpha

Background

ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ROCK2
Entrez
Uniprot