ROCK2 Antibody - BSA Free

Western Blot: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL

Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody - BSA Free [NBP1-76570] - Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 ug/mL.

Immunohistochemistry: ROCK2 Antibody - BSA Free [NBP1-76570] - Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ROCK2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ROCK2 Antibody - BSA Free (NBP1-76570) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2. The immunogen is located within amino acids 920 - 970 of ROCK2. Amino Acid Squence: DLEKEKIMKELEIKE
• Specificity: Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ROCK2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ROCK2 Peptide (NBP1-76570PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ROCK2 Antibody - BSA Free

• coiled-coil-containing protein kinase 2
• EC 2.7.11
• EC 2.7.11.1
• KIAA0619
• p164 ROCK-2
• Rho kinase 2
• rho-associated protein kinase 2
• Rho-associated, coiled-coil containing protein kinase 2
• ROCK2
• ROCK-II
• ROKalpha

Background

ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ROCK2
• Entrez:
  • 9475(Human)
• Uniprot:
  • O75116()