RNF70 Antibody - BSA Free

Images

 
Western Blot: RNF70 Antibody [NBP2-81998] - Analysis of RNF70 in human brain tissue lysate with RNF70 antibody at (A) 0.25 and (B) 0.5 ug/ml.
Immunohistochemistry: RNF70 Antibody - BSA Free [NBP2-81998] - Immunohistochemistry of RNF70 in mouse brain tissue with RNF70 antibody at 5 u/ml.
Immunocytochemistry/ Immunofluorescence: RNF70 Antibody - BSA Free [NBP2-81998] - Immunofluorescence of RNF70 in mouse brain tissue with RNF70 antibody at 20 u/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

RNF70 Antibody - BSA Free Summary

Immunogen
RNF70 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF70. The immunogen is located within amino acids 50 - 100 of RNF70. Amino Acid Squence: QTTKRSRSPFSTTRRSWDD
Specificity
RNF70 antibody is human, mouse and rat reactive. At least two isoforms of RNF70 are known to exist; this antibody will detect both isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PJA1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 0.25-0.5 ug/ml
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RNF70 Antibody - BSA Free

  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ11830
  • praja 1
  • praja ring finger 1
  • PRAJA1
  • RING finger protein 70
  • RNF70E3 ubiquitin-protein ligase Praja-1

Background

RNF70 has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequentdegradation by proteosome . May be involved in protein sorting

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PJA1