RNF70 Antibody - BSA Free

Western Blot: RNF70 Antibody [NBP1-87399] - Analysis in human cell line RT-4.

Immunocytochemistry/Immunofluorescence: RNF70 Antibody [NBP1-87399] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.

Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399] - Staining in human cerebral cortex and liver tissues using anti-PJA1 antibody. Corresponding PJA1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399] - Staining of human esophagus shows nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: RNF70 Antibody [NBP1-87399] - Staining of human liver shows low expression as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RNF70 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PJA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RNF70 Recombinant Protein Antigen (NBP1-87399PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RNF70 Antibody - BSA Free

• EC 6.3.2
• EC 6.3.2.-
• FLJ11830
• praja 1
• praja ring finger 1
• PRAJA1
• RING finger protein 70
• RNF70E3 ubiquitin-protein ligase Praja-1

Background

RNF70 has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequentdegradation by proteosome . May be involved in protein sorting

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PJA1