RNF34 Antibody

Product Discontinued

RNF34 Antibody Summary

• Immunogen: Peptide with sequence C-RLYKENEENQKSY corresponding to internal region according to NP_079402.2.
• Epitope: C-RLYKENEENQKSY
• Specificity: This antibody is expected to recognize both reported isoforms (NP_919247.1; NP_079402.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF34
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 35 kDa band in human lymph node, ovary and testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.7 kDa band according to NP_919247.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  RNF34 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for RNF34 Antibody

• CARP1
• Caspase regulator CARP1
• Caspases-8 and -10-associated RING finger protein 1
• E3 ubiquitin-protein ligase RNF34
• EC 6.3.2
• EC 6.3.2.-
• FLJ21786
• FYVE-RING finger protein Momo
• hRFI
• Human RING finger homologous to inhibitor of apoptosis protein
• RFI
• RIF
• RIFF
• ring finger protein 34CARP-1
• RING finger protein RIFF

Background

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF34 Antibody (NB100-93460)(1)

Publication using NB100-93460

• Konishi T, Sasaki S, Watanabe T et al. Overexpression of hRFI (human ring finger homologous to inhibitor of apoptosis protein type) inhibits death receptor-mediated apoptosis in colorectal cancer cells. Mol Cancer Ther 2005-05-01 [PMID: 15897238]

Bioinformatics

• Gene Symbol: RNF34
• Entrez:
  • 80196(Human)
  • 80751(Mouse)
  • 282845(Rat)
• Uniprot:
  • NP_919247.1(Human)