RNF20 Antibody - BSA Free

Western Blot: RNF20 Antibody [NB100-2243] - Detection of Human RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2243 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: RNF20 Antibody [NB100-2243] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2243 used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by rabbit anti-RNF20 antibody NB100-2242. For blotting immunoprecipitated RNF20, NB100-2243 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Bv, Ch
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RNF20 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RNF20 Antibody - BSA Free (NB100-2243) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
• Predicted Species: Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2- 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF20 Antibody - BSA Free

• BRE1 E3 ubiquitin ligase homolog
• BRE1
• BRE1-A
• BRE1AMGC129667
• E3 ubiquitin-protein ligase BRE1A
• EC 6.3.2
• EC 6.3.2.-
• FLJ11189
• FLJ20382
• hBRE1MGC129668
• homolog of S. cerevisiae BRE1
• KAIA2779
• ring finger protein 20KIAA2779

Background

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription. Alternate names for RNF20 include E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, FLJ11189, FLJ20382, KIAA2779, MGC129667, and MGC129668.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF20
• Entrez:
  • 56254(Human)
• Uniprot:
  • Q5VTR2(Human)