RNF20 Antibody

Images

 
Western Blot: RNF20 Antibody [NB100-2243] - Detection of Human RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: RNF20 Antibody [NB100-2243] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: RNF20 Antibody [NB100-2243] - Detection of Human RNF20 on HeLa whole cell lysate using NB100-2243.
Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-RNF20 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of human prostate carcinoma. Affinity purified rabbit anti-RNF20 used at a dilution of 1:250.
Immunohistochemistry: RNF20 Antibody [NB100-2243] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: RNF20 Antibody [NB100-2243] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

RNF20 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF20
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF20 Antibody

  • BRE1 E3 ubiquitin ligase homolog
  • BRE1
  • BRE1-A
  • BRE1AMGC129667
  • E3 ubiquitin-protein ligase BRE1A
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ11189
  • FLJ20382
  • hBRE1MGC129668
  • homolog of S. cerevisiae BRE1
  • KAIA2779
  • ring finger protein 20KIAA2779

Background

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription. Alternate names for RNF20 include E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, FLJ11189, FLJ20382, KIAA2779, MGC129667, and MGC129668.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RNF20
Entrez
Uniprot