RNF40 Antibody

Images

 
Western Blot: RNF40 Antibody [NBP2-20189] - RNF40 antibody detects RNF40 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was ...read more
Immunocytochemistry/ Immunofluorescence: RNF40 Antibody [NBP2-20189] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RNF40 Antibody [NBP2-20189] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: RNF40 Antibody [NBP2-20189] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunoprecipitation: RNF40 Antibody [NBP2-20189] - Immunoprecipitation of RNF40 protein from HeLa whole cell extracts using 5 ug of RNF40 antibody. Western blot analysis was performed using RNF40 antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RNF40 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RNF40. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF40
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for RNF40 Antibody

  • 95 kDa retinoblastoma-associated protein
  • BRE1-B
  • BRE1BMGC13051
  • EC 6.3.2,95 kDa retinoblastoma protein binding protein
  • EC 6.3.2.-
  • KIAA0661BRE1 E3 ubiquitin ligase homolog B
  • Rb-associated protein
  • RBP95E3 ubiquitin-protein ligase BRE1B
  • ring finger protein 40DKFZp686K191
  • STARING

Background

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF40
Uniprot