RNF20 Antibodies
We offer RNF20 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each RNF20 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RNF20 Antibodies can be used in a variety of model species: Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat. Use the list below to choose the RNF20 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RNF20 polyclonal antibodies and browse our RNF20 monoclonal antibody catalog.
Alternate Names for RNF20 Antibodies
anti-RNF20 antibody, anti-BRE1 antibody, anti-BRE1 E3 ubiquitin ligase homolog antibody, anti-BRE1-A antibody, anti-BRE1AMGC129667 antibody, anti-E3 ubiquitin-protein ligase BRE1A antibody, anti-EC 6.3.2 antibody, anti-EC 6.3.2.- antibody, anti-FLJ11189 antibody, anti-FLJ20382 antibody, anti-hBRE1MGC129668 antibody, anti-homolog of S. cerevisiae BRE1 antibody, anti-KAIA2779 antibody, anti-ring finger protein 20KIAA2779 antibody
12 Results for "rnf20" in Primary Antibodies
![Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NB100-2242-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: RNF20 Antibody [NB100-2242] - WB analysis of RNF20 in HeLa whole cell lysate. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NB100-2242-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RNF20 Antibody [NB100-2242] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2242 (lot NB100-2242-2) used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by a previous lot of this antibody (NB100-2242-1) and rabbit anti-RNF20 antibody NB100-2243. For blotting immunoprecipitated RNF20, NB100-2242 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RNF20-Antibody-Immunoprecipitation-NB100-2242-img0007.jpg)
![Western Blot: RNF20 Antibody [NB100-2243] - Detection of Human RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2243 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NB100-2243-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: RNF20 Antibody [NB100-2243] - Detection of Human RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2243 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNF20-Antibody-Immunohistochemistry-NB100-2243-img0006.jpg)
![Immunohistochemistry-Paraffin: RNF20 Antibody [NB100-2243] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- RNF20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RNF20-Antibody-Immunohistochemistry-NB100-2243-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RNF20 Antibody [NB100-2243] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2243 used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by rabbit anti-RNF20 antibody NB100-2242. For blotting immunoprecipitated RNF20, NB100-2243 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RNF20-Antibody-Immunoprecipitation-NB100-2243-img0010.jpg)
![Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-262202414225224.jpg "Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-26220241525337.jpg)
![Western Blot: RNF20 Antibody (2H12) [NBP3-26341] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate. All lanes: RNF20 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-282202411472147.jpg)
![Immunocytochemistry/Immunofluorescence: RNF20 Antibody (2H12) [NBP3-26341] - Staining of Hela Cells with RNF20 Antibody (2H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26341_rabbit-rnf20-mab-2h12-282202412475144.jpg)
![Western Blot: RNF20 Antibody [NBP3-27887] - Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 u/mL.](http://images.novusbio.com/fullsize/nbp3-27887_rabbit-rnf20-pab-2711202420405533.gif "Western Blot: RNF20 Antibody [NBP3-27887] - Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 u/mL.")
![Immunohistochemistry: RNF20 Antibody [NBP3-27887] - Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 u/ml.](http://images.novusbio.com/fullsize/nbp3-27887_rabbit-rnf20-pab-2711202419562136.jpg)
![Immunocytochemistry/ Immunofluorescence: RNF20 Antibody [NBP3-27887] - Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp3-27887_rabbit-rnf20-pab-271120241937756.jpg)
![Western Blot: RNF20 Antibody (1E4) [H00056254-M02] - Detection against Immunogen (36.74 KDa) .](http://images.novusbio.com/fullsize/RNF20-Antibody-1E4-Western-Blot-H00056254-M02-img0001.jpg "Western Blot: RNF20 Antibody (1E4) [H00056254-M02] - Detection against Immunogen (36.74 KDa) .")
![Immunocytochemistry/Immunofluorescence: RNF20 Antibody [NBP2-54995] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RNF20-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54995-img0001.jpg "Immunocytochemistry/Immunofluorescence: RNF20 Antibody [NBP2-54995] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Western Blot: RNF20 Antibody [NBP1-52985] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NBP1-52985-img0002.jpg "Western Blot: RNF20 Antibody [NBP1-52985] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: RNF20 Antibody [NBP1-52945] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NBP1-52945-img0002.jpg "Western Blot: RNF20 Antibody [NBP1-52945] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.")
![Sandwich ELISA: RNF20 Antibody (1C6) [H00056254-M01] - Detection limit for recombinant GST tagged RNF20 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RNF20-Antibody-1C6-Sandwich-ELISA-H00056254-M01-img0002.jpg "Sandwich ELISA: RNF20 Antibody (1C6) [H00056254-M01] - Detection limit for recombinant GST tagged RNF20 is approximately 0.03ng/ml as a capture antibody.")