RNF12 Antibody

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Western Blot: RNF12 Antibody [NBP2-20175] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.66 mg/ml

Order Details

RNF12 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RNF12. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RLIM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (86%), Rat (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.66 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF12 Antibody

  • DKFZp686N06224
  • E3 ubiquitin-protein ligase RLIM
  • E3 ubiquitin-protein ligase RNF12
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ25923
  • FLJ41093
  • LIM domain-interacting RING finger protein
  • MGC15161
  • NY-REN-43
  • Renal carcinoma antigen NY-REN-43
  • RING finger LIM domain-binding protein
  • RING finger protein 12FLJ42887
  • ring finger protein, LIM domain interacting
  • ring zinc finger LIM domain binding protein
  • ring zinc finger protein NY-REN-43antigen
  • R-LIM
  • RNF12FLJ45986

Background

The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RLIM
Uniprot