RNF12 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: RNF12 Antibody [NBP1-89571] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: RNF12 Antibody [NBP1-89571] - Staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RNF12 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:RSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDL
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RLIM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RNF12 Protein (NBP1-89571PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RNF12 Antibody

  • DKFZp686N06224
  • E3 ubiquitin-protein ligase RLIM
  • E3 ubiquitin-protein ligase RNF12
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ25923
  • FLJ41093
  • LIM domain-interacting RING finger protein
  • MGC15161
  • NY-REN-43
  • Renal carcinoma antigen NY-REN-43
  • RING finger LIM domain-binding protein
  • RING finger protein 12FLJ42887
  • ring finger protein, LIM domain interacting
  • ring zinc finger LIM domain binding protein
  • ring zinc finger protein NY-REN-43antigen
  • R-LIM
  • RNF12FLJ45986

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RLIM