RNF12 Antibody - BSA Free

Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RNF12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RNF12 Antibody - BSA Free (NBP1-89571) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RLIM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RNF12 Protein (NBP1-89571PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RNF12 Antibody - BSA Free

• DKFZp686N06224
• E3 ubiquitin-protein ligase RLIM
• E3 ubiquitin-protein ligase RNF12
• EC 6.3.2
• EC 6.3.2.-
• FLJ25923
• FLJ41093
• LIM domain-interacting RING finger protein
• MGC15161
• NY-REN-43
• Renal carcinoma antigen NY-REN-43
• RING finger LIM domain-binding protein
• RING finger protein 12FLJ42887
• ring finger protein, LIM domain interacting
• ring zinc finger LIM domain binding protein
• ring zinc finger protein NY-REN-43antigen
• R-LIM
• RNF12FLJ45986

Background

RNF12 is encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RLIM