RNase H1 Antibody - BSA Free

Western Blot: RNase H1 Antibody [NBP2-20171] - Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] (NBP2-20171) diluted at 1:1000.

Immunohistochemistry-Paraffin: RNase H1 Antibody [NBP2-20171] - Human breast cancer, using RNase H1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RNase H1 antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Ze, Ca, Pm, Rb, RM
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RNase H1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RNase H1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rabbit (91%), Chimpanzee (100%), Rhesus Macaque (95%), Canine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNASEH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Reported in scientific literature (PMID: 26106861)
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34937926). Bovine (87%), Guinea pig (87%), Pig (87%), Sheep (87%), Rat (89%). .

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RNase H1 Antibody - BSA Free

• EC 3.1.26.4
• H1RNA
• Ribonuclease H type II
• ribonuclease H1
• RNase H1
• RNH1

Background

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RNase H1 Antibody (NBP2-20171)(2)

Publications using NBP2-20171

• Shukla V, Samaniego-Castruita D, Dong Z et al. TET deficiency perturbs mature B cell homeostasis and promotes oncogenesis associated with accumulation of G-quadruplex and R-loop structures Nature immunology 2022-01-01 [PMID: 34937926] (WB, Mouse)
• Tresini M, Warmerdam DO, Kolovos P et al. The core spliceosome as target and effector of non-canonical ATM signalling. Nature 2015-07-02 [PMID: 26106861] (IP, Human)

Bioinformatics

• Gene Symbol: RNASEH1
• Uniprot:
  • O60930(Human)