RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 8A7) - BSA Free

Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 8A7) [NBP2-50243] - RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNAP II is reversibly phosphorylated on the highly conserved heptapeptide repeat, YSPTSPS. The major physiological phosphorylation sites are Ser-2 and Ser-5. Monoclonal antibody 8A7 was obtained using synthetic phosphopeptides and specifically recognises the Ser-5 phosphorylated form of RNAP II.

• Reactivity: Hu, Ye
• Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: CTD 8A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 8A7) - BSA Free Summary

• Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically sythesized phospho-Ser5.
• Modification: p Ser5
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2A
• Purity: Thiophilic Chromatography

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is Cytof ready. Positive control(s): S. cerevisiae and HeLa cells.

Reactivity Notes

Saccharomyces cerevisiae

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Thiophilic Chromatography

Alternate Names for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 8A7) - BSA Free

• DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
• DNA-directed RNA polymerase II subunit A
• DNA-directed RNA polymerase II subunit RPB1
• DNA-directed RNA polymerase III largest subunit
• EC 2.7.7.48
• EC 2.7.7.6
• hRPB220
• hsRPB1
• MGC75453
• POLR2
• POLR2A
• POLRA
• polymerase (RNA) II (DNA directed) polypeptide A (220kD)
• polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• RNA Pol 2
• RNA Polymerase 2
• RNA polymerase II subunit B1
• RNA Polymerase II
• RNA-directed RNA polymerase II subunit RPB1
• RNAPII
• RPB1
• RPBh1
• RpIILS
• RPO2
• RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2A