RNA Polymerase II/POLR2A Antibody (4H8) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Mu, Ye, Pm(-), Drosophila, Hamster, PlantSpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF, IP
Clone
4H8
Clonality
Monoclonal
Host
Mouse

RNA Polymerase II/POLR2A Antibody (4H8) [DyLight 350] Summary

Immunogen
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
Specificity
This antibody recognizes the C-terminal repeat of the largest subunit of RNA polymerase II. It recognizes both unphosphorylated and phosphorylated forms.
Predicted Species
Hamster (100%), Drosophila (100%), Plant (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POLR2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 350
Format
DyLight 350 (A=353, E=432)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
This RNA polymerase II Antibody (4H8) is useful for Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation and Western blot. In WB a band can be seen at approximately 217 kDa. Optimal dilutions/concentrations should be determined by the end user.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
217 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, S. cerevisiae (Yeast), Mouse. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology - all of these species contain at least one or more 100% identical repeats. Does not cross react with monkey.

Alternate Names for RNA Polymerase II/POLR2A Antibody (4H8) [DyLight 350]

  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II subunit RPB1
  • DNA-directed RNA polymerase III largest subunit
  • EC 2.7.7.48
  • EC 2.7.7.6
  • hRPB220
  • hsRPB1
  • MGC75453
  • POLR2
  • POLR2A
  • POLRA
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • RNA Pol 2
  • RNA Polymerase 2
  • RNA polymerase II subunit B1
  • RNA Polymerase II/POLR2A
  • RNA-directed RNA polymerase II subunit RPB1
  • RNAPII
  • RPB1
  • RPBh1
  • RpIILS
  • RPO2
  • RPOL2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for RNA Polymerase II/POLR2A Antibody (NB200-598UV) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB200-598APC
Biotin Labeled NB200-598B
DyLight 405 Labeled NB200-598V
DyLight 405LS Labeled NB200-598V3
DyLight 488 Labeled NB200-598G
DyLight 550 Labeled NB200-598R
DyLight 650 Labeled NB200-598C
DyLight 680 Labeled NB200-598FR
DyLight 755 Labeled NB200-598IR
FITC Labeled NB200-598F
HRP Labeled NB200-598H
PE Labeled NB200-598PE
PerCP Labeled NB200-598PCP

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol POLR2A
Entrez
Uniprot

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