RNA Helicase A Antibody

Western Blot: RNA Helicase A Antibody [NBP3-12975] - RNA Helicase A antibody detects RNA Helicase A protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RNA Helicase A antibody (NBP3-12975) diluted at 1:1000.

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP3-12975] - RNA Helicase A antibody detects RNA Helicase A protein at cytoplasm and nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. RNA Helicase A antibody (NBP3-12975) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NBP3-12975] - RNA Helicase A antibody detects RNA Helicase A protein at cytoplasm and nucleus in A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. RNA Helicase A antibody (NBP3-12975) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

DHX9 antibody detects DHX9 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DHX9 protein stained by DHX9 antibody (NBP3-12975) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RNA Helicase A Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RNA Helicase A. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RNA Helicase A Antibody

• DDX9
• DDX9ATP-dependent RNA helicase A
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNAhelicase II; leukophysin)
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9
• DEAH (Asp-Glu-Ala-His) box polypeptide 9
• DEAH box protein 9
• DExH-box helicase 9
• DHX9
• EC 3.6.1
• EC 3.6.1.15
• EC 3.6.4.13
• FLJ17406
• Leukophysin
• LKP
• NDH II
• NDH2
• NDHII
• Nuclear DNA helicase II
• RHA
• RNA Helicase A

Background

DHX9, also known as ATP-dependent RNA helicase A, consists of a 141 kDa and a 24.3 kDa isoform and is involved in activating transcription and unwinding DNA and RNA helixes in the 3' to 5' direction. Current research is being conducted on diseases and disorders such as influenza, hepatitis, t-cell leukemia, astigmatism, keratitis, immunodeficiency, Werner syndrome, and arthritis. The protein interacts in mRNA splicing, mRNA processing, and gene expression pathways with HIST1H3A- HIST13E proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX9