RNA Helicase A Antibody (JE34-97)

Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunocytochemistry analysis of HeLa cells labeling RNA Helicase A with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: JE34-97
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RNA Helicase A Antibody (JE34-97) Summary

• Description: Novus Biologicals Rabbit RNA Helicase A Antibody (JE34-97) (NBP3-32907) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human RNA Helicase A aa 1,126-1,225 / 1,270. (Uniprot: Q08211)
• Localization: Nucleus, nucleoplasm, nucleolus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosom.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DHX9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:1000-1:2000
• Theoretical MW: 141 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RNA Helicase A Antibody (JE34-97)

• DDX9
• DDX9ATP-dependent RNA helicase A
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNAhelicase II; leukophysin)
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9
• DEAH (Asp-Glu-Ala-His) box polypeptide 9
• DEAH box protein 9
• DExH-box helicase 9
• DHX9
• EC 3.6.1
• EC 3.6.1.15
• EC 3.6.4.13
• FLJ17406
• Leukophysin
• LKP
• NDH II
• NDH2
• NDHII
• Nuclear DNA helicase II
• RHA
• RNA Helicase A

Background

DHX9, also known as ATP-dependent RNA helicase A, consists of a 141 kDa and a 24.3 kDa isoform and is involved in activating transcription and unwinding DNA and RNA helixes in the 3' to 5' direction. Current research is being conducted on diseases and disorders such as influenza, hepatitis, t-cell leukemia, astigmatism, keratitis, immunodeficiency, Werner syndrome, and arthritis. The protein interacts in mRNA splicing, mRNA processing, and gene expression pathways with HIST1H3A- HIST13E proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX9