RIPK3/RIP3 Antibody (780115) [Unconjugated]

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody ...read more
RIPK3/RIP3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) at 15 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
780115
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

RIPK3/RIP3 Antibody (780115) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human RIPK3/RIP3
Met1-Arg218
Accession # Q9Y572
Specificity
Detects human RIPK3/RIP3 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RIPK3
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB7604 in the following applications:

Publications
Read Publications using
MAB7604 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIPK3/RIP3 Antibody (780115) [Unconjugated]

  • EC 2.7.11.1
  • Receptor Interacting Serine/Threonine Kinase 3
  • Receptor-interacting protein 3
  • receptor-interacting serine/threonine-protein kinase 3
  • receptor-interacting serine-threonine kinase 3
  • RIP3
  • RIP-3
  • RIPK3
  • RIP-like protein kinase 3

Background

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, IHC

Publications for RIPK3/RIP3 Antibody (MAB7604)(3)

Review for RIPK3/RIP3 Antibody (MAB7604) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB7604:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western RIPK3/RIP3 MAB7604
Enlarge
5
reviewed by:
Shruti Lal
Simple Western Human 10/16/2020
View

Summary

ApplicationSimple Western
Sample TestedHT-29 colon cancer cell line
SpeciesHuman
LotCGTV0119091

Comments

Comments*Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RIPK3/RIP3 Antibody (MAB7604). (Showing 1 - 3 of 3 FAQs).

  1. What research areas can this product be used in?
    • All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.
  2. What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?
    • This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
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1
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1
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Shruti Lal
10/16/2020
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol RIPK3
Uniprot