RIPK3/RIP3 Antibody (780115) [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RIPK3/RIP3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 780115
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

RIPK3/RIP3 Antibody (780115) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human RIPK3/RIP3
  Met1-Arg218
  Accession # Q9Y572
• Specificity: Detects human RIPK3/RIP3 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIPK3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIPK3/RIP3 Antibody (780115) [Unconjugated]

• EC 2.7.11.1
• Receptor Interacting Serine/Threonine Kinase 3
• Receptor-interacting protein 3
• receptor-interacting serine/threonine-protein kinase 3
• receptor-interacting serine-threonine kinase 3
• RIP3
• RIP-3
• RIPK3
• RIP-like protein kinase 3

Background

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RIPK3/RIP3 Antibody (MAB7604)(4)

Publications using MAB7604

• Zhou, B;Xiao, K;Guo, J;Xu, Q;Xu, Q;Lv, Q;Zhu, H;Zhao, J;Liu, Y; Necroptosis contributes to the intestinal toxicity of deoxynivalenol and is mediated by methyltransferase SETDB1 Journal of hazardous materials 2024-08-05 [PMID: 38823098] (Western Blot, Porcine)
• YA Kung, HJ Chiang, ML Li, YN Gong, HP Chiu, CT Hung, PN Huang, SY Huang, PY Wang, TA Hsu, G Brewer, SR Shih Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis MBio, 2022-01-18;0(0):e0271721. 2022-01-18 [PMID: 35038927] (Western Blot, Human)
• R Cheng, X Liu, Z Wang, K Tang ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis Molecular Medicine Reports, 2021-03-31;23(6):. 2021-03-31 [PMID: 33786632] (Co-Immunoprecipitation, Western Blot, Human)
• J Kim, JY Chung, YS Park, SJ Jang, HR Kim, CM Choi, JS Song Prognostic Significance of CHIP and RIPK3 in Non-Small Cell Lung Cancer Cancers (Basel), 2020-06-08;12(6):. 2020-06-08 [PMID: 32521727] (IHC, Human)

Review for RIPK3/RIP3 Antibody (MAB7604) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 10/16/2020

Summary

• Application: Simple Western
• Sample Tested: HT-29 colon cancer cell line
• Species: Human
• Lot: CGTV0119091

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

FAQs for RIPK3/RIP3 Antibody (MAB7604). (Showing 1 - 3 of 3 FAQs).

1. What research areas can this product be used in?
   • All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.
2. What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?
   • This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.
3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

Bioinformatics

• Gene Symbol: RIPK3
• Uniprot:
  • Q9Y572(Human)