RIPK2 Antibody - BSA Free

Western Blot: RIPK2 Antibody [NBP1-76960] - Analysis of RIPK2 in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RIPK2 antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: RIPK2 Antibody [NBP1-76960] - K562 cells with RIPK2 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody - BSA Free [NBP1-76960] - Immunofluorescence of RIPK2 in K562 cells with RIPK2 antibody at 20 ug/mL.Red: RIPK2 Antibody Blue: DAPI staining

Immunocytochemistry/ Immunofluorescence: RIPK2 Antibody - BSA Free [NBP1-76960] - Immunocytochemistry of RIPK2 in A431 cells with RIPK2 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RIPK2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK. The immunogen is located within the first 50 amino acids of RICK. Amino Acid Squence: PTIPYHKLADLRYLSRGASG
• Predicted Species: Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RIPK2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/mL
  • Western Blot 1 ug/mL
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  RIPK2 Peptide (NBP1-76960PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RIPK2 Antibody - BSA Free

• CARD3
• CARD-containing IL-1 beta ICE-kinase
• CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase
• CARD-containing interleukin-1 beta-converting enzyme-associated kinase
• CARDIAKCCK
• EC 2.7.11
• EC 2.7.11.1
• GIG30
• growth-inhibiting gene 30
• receptor interacting protein 2
• receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase
• Receptor-interacting protein 2
• receptor-interacting serine/threonine-protein kinase 2
• receptor-interacting serine-threonine kinase 2
• RICK
• RICKGIG30
• RIP2
• RIP-2
• RIP2CARD-carrying kinase
• RIPK2
• RIP-like-interacting CLARP kinase

Background

Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-kappaB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1beta. The messenger RNA of RICK is expressed in multiple human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RIPK2 Antibody (NBP1-76960)(1)

Publication using NBP1-76960

• Browne CM, Jiang B, Ficarro SB et al. A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification J. Am. Chem. Soc. 2019-01-09 [PMID: 30518210] (WB, Human)

Bioinformatics

• Gene Symbol: RIPK2
• Uniprot:
  • O43353()