Cholecystokinin-B R/CCKBR Antibody

Images

 
Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody
Immunohistochemistry-Paraffin: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining
Immunohistochemistry-Paraffin: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Staining of paraffin embedded Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Cholecystokinin-B R/CCKBR Antibody Summary

Immunogen
Peptide with sequence C-FDGDSDSDSQSRVRNQ corresponding to internal region according to NP_795344.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCKBR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 4-6 ug/ml
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
ICC/IF successful usage for RIN-14B cells is reported in scientific literature (PMID: 19959964)and for rat pancreatic stellate cells is reported in scientific literature (PMID: 20843811). Data obtained from a previous batch (different goat).
WB: Approx. 75 kDa band observed in purified membranes of rat insulinoma cell line RIN14B (PMID: 20011057). The observed MW also corresponds to earlier findings in scientific literature with different antibodies (PMID: 14566022). Data obtained from a previous batch (different goat).
IHC-P: Human pancreas shows staining of the islets of langerhans.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-2803.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cholecystokinin-B R/CCKBR Antibody

  • CCK2 receptor
  • CCK2R
  • CCK2-R
  • CCK-B receptor
  • CCK-B
  • CCKBR
  • CCK-BR
  • CCKRB
  • cholecystokinin B receptor
  • Cholecystokinin-2 receptor
  • Cholecystokinin-B R
  • cholecystokinin-B receptor/gastrin receptor
  • CholecystokininBR
  • Cholecystokinin-BR
  • GASR
  • Gastrin Receptor
  • gastrin/cholecystokinin type B receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cholecystokinin-B R/CCKBR Antibody (NB100-2803)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CCKBR
Entrez
Uniprot