RIPK2 Antibody (1018218) [Unconjugated]

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103871) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RIPK2/RIP2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103871) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103871) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 1018218
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RIPK2 Antibody (1018218) [Unconjugated] Summary

• Immunogen: E. coli-derived human RIPK2/RIP2
  Met1-Met540
  Accession # O43353
• Specificity: Detects human RIPK2/RIP2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIPK2 Antibody (1018218) [Unconjugated]

• CARD3
• CARD-containing IL-1 beta ICE-kinase
• CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase
• CARD-containing interleukin-1 beta-converting enzyme-associated kinase
• CARDIAKCCK
• EC 2.7.11
• EC 2.7.11.1
• GIG30
• growth-inhibiting gene 30
• receptor interacting protein 2
• receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase
• Receptor-interacting protein 2
• receptor-interacting serine/threonine-protein kinase 2
• receptor-interacting serine-threonine kinase 2
• RICK
• RICKGIG30
• RIP2
• RIP-2
• RIP2CARD-carrying kinase
• RIPK2
• RIP-like-interacting CLARP kinase

Background

Receptor-interacting serine/threonine-protein kinase 2, also known as RIPK2 or RIP-2, is a  540 amino acids (aa) Serine/threonine/tyrosine kinase encoded by the RIPK2 gene. RIP-2, a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, plays an essential role in modulation of innate and adaptive immune responses. Human RIP2 is an adapter protein necessary for signal propagation of the Nucleotide-binding-oligomerization-domain-containing proteins 1/2 (NOD1 and NOD2). Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through the C-terminal caspase recruitment domain (CARD). Human and mouse RIPK2 aa sequences are 84% identical .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O43353()