CCK-AR Antibody [Unconjugated]

CCK-A R was detected in perfusion fixed frozen sections of rat pancreas using 5 µg/mL Human/Mouse/Rat CCK-A R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2680) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Product Discontinued

CCK-AR Antibody [Unconjugated] Summary

• Immunogen: Rat CCK-A R synthetic peptide
  Lys256-Glu267
  Accession # P30551
• Specificity: Detects human, mouse, and rat CCK-A R.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCKAR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCK-AR Antibody [Unconjugated]

• CCK1R
• CCK1-R
• CCK-A R
• CCK-A receptor
• CCK-A
• CCKAR
• CCK-AR
• CCKRA
• cholecystokinin A receptor
• cholecystokinin receptor type A
• cholecystokinin type-A receptor
• Cholecystokinin-1 receptor

Background

The physiologic actions of the cholecystokinin (CCK) family of peptide hormones are mediated through two classes of G protein-coupled receptors, CCK-A R and CCK-B R. CCK-A R is a 428 amino acid protein with 7 putative transmembrane domains. This GPCR is involved in gallbladder contraction, insulin secretion, and has also been described in specific regions of the central nervous system. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCK-AR Antibody (AF2680)(3)

Publications using AF2680

• He L, Shi H, Zhang G et al. A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition The Journal of Neuroscience 2023-03-29 [PMID: 36813575]
• He L, Shi H, Zhang G et al. A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition The Journal of Neuroscience 3/29/2023 [PMID: 36813575]
• Zheng Z, Lewis MW, Travagli RA In vitro analysis of the effects of cholecystokinin on rat brain stem motoneurons. Am. J. Physiol. Gastrointest. Liver Physiol., 2004-12-09;288(5):G1066-73. 2004-12-09 [PMID: 15591159] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: CCKAR
• Uniprot:
  • P30551()