![Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human cerebral cortex, colon, liver and tonsil using Anti-RPN1 antibody NBP2-55523 (A) shows similar protein distribution across tissues to independent antibody NBP1-82467 (B).](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Immunohistochemistry-Paraffin-NBP2-55523-img0004.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human cerebral cortex, colon, liver and tonsil using Anti-RPN1 antibody NBP2-55523 (A) shows similar protein distribution across tissues to independent antibody NBP1-82467 (B).")
| Reactivity | Hu, Rt, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Ribophorin I Antibody - BSA Free (NBP2-55523) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Ribophorin I Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQ |
| Predicted Species | Mouse (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RPN1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC Retrieval method: HIER pH6. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunocytochemistry_6580.jpg)
![Immunohistochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunohistochemistry_22800.jpg)
![Neutralization CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Block_Neutralize_9048.jpg)
| Publication using NBP2-55523 | Applications | Species |
|---|---|---|
| Luo F, Smagris E, Martin S et al. Hepatic TM6SF2 is Required for Lipidation of VLDL in a Pre-Golgi Compartment in Mice and Rats Cellular and molecular gastroenterology and hepatology 2021-12-16 [PMID: 34923175] (WB, Rat) | WB | Rat |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RPN1 |
![Western Blot: Ribophorin I Antibody [NBP2-55523] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Western-Blot-NBP2-55523-img0002.jpg "Western Blot: Ribophorin I Antibody [NBP2-55523] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody [NBP2-55523] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55523-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody [NBP2-55523] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human liver.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Immunohistochemistry-Paraffin-NBP2-55523-img0007.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human liver.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human tonsil.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Immunohistochemistry-Paraffin-NBP2-55523-img0005.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human tonsil.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human colon.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Immunohistochemistry-Paraffin-NBP2-55523-img0006.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human colon.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-Immunohistochemistry-Paraffin-NBP2-55523-img0008.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human cerebral cortex.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")