ADRM1 Antibody - BSA Free

Immunohistochemistry: ADRM1 Antibody [NBP3-38097] - Immunohistochemistry analysis of paraffin-embedded Rat stomach using ADRM1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: ADRM1 Antibody [NBP3-38097] - Western blot analysis of various lysates using ADRM1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Immunohistochemistry: ADRM1 Antibody [NBP3-38097] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ADRM1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: ADRM1 Antibody [NBP3-38097] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ADRM1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: ADRM1 Antibody [NBP3-38097] - Immunofluorescence analysis of U2OS cells using ADRM1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: ADRM1 Antibody [NBP3-38097] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ADRM1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ADRM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ADRM1 Antibody - BSA Free (NBP3-38097) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 108-407 of human ADRM1 (NP_008933.2).
  
  Sequence:
  WMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADRM1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:1000 - 1:4000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ADRM1 Antibody - BSA Free

• 000 surface antigen
• 110 kDa cell membrane glycoprotein
• adhesion regulating molecule 1
• Adhesion-regulating molecule 1
• ADRM1
• ARM1
• ARM-1
• Gp110
• GP110hRpn13
• M(r) 110
• MGC29536
• proteasomal ubiquitin receptor ADRM1
• Proteasome regulatory particle non-ATPase 13
• PSMD16
• regulatory particle non-ATPase 13
• Rpn13 homolog
• Rpn13

Background

ADRM1 is encoded by this gene is an integral plasma membrane protein which promotes cell adhesion. The encoded protein is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADRM1