Ribophorin I Antibody

Images

 
Western Blot: Ribophorin I Antibody [NBP1-32704] - Sample(30 ug of whole cell lysate)A:H1299 B:Hep G2 10% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody [NBP1-32704] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Ribophorin I Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Ribophorin I. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane, Single-pass type I membrane protein, Melanosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
68.57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribophorin I Antibody

  • DKFZp686B16177
  • dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
  • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
  • EC 2.4.1.119
  • oligosaccharyltransferase 1 homolog
  • OST1
  • ribophorin IRBPH1
  • ribophorin-1
  • RPN-I

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPN1
Entrez
Uniprot