Ribophorin I Antibody - BSA Free

Western Blot: Ribophorin I Antibody [NBP1-32704] - Sample(30 ug of whole cell lysate)A:H1299 B:Hep G2 10% SDS PAGE, antibody diluted at 1:1500.

Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody [NBP1-32704] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP1-32704] - Paraffin-embedded human normal kidney. Ribophorin I antibody [C2C3], C-term diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ribophorin I Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ribophorin I Antibody - BSA Free (NBP1-32704) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Ribophorin I. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane, Single-pass type I membrane protein , Melanosome
• Predicted Species: Mouse (96%), Rat (97%), Porcine (96%), Chimpanzee (99%), Bovine (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Ribophorin I Antibody - BSA Free

• DKFZp686B16177
• dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
• Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
• dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• EC 2.4.1.119
• oligosaccharyltransferase 1 homolog
• OST1
• ribophorin IRBPH1
• ribophorin-1
• RPN-I

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RPN1
• Entrez:
  • 6184(Human)
• Uniprot:
  • P04843(Human)