Ribophorin I Antibody (OTI3H3) - Azide and BSA Free

Western Blot: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribophori

Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Expression of RPN1 on pica cell lines vs normal. Image from verified customer review.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.

Flow Cytometry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - HEK293T cells transfected with either Ribophorin I Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RPN1 antibody , and then analyzed by flow cytometry.

Western Blot: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.

Immunohistochemistry: Ribophorin I Antibody (OTI3H3) - Azide and BSA Free [NBP2-73898] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Ribophorin I Antibody (OTI3H3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Ribophorin I Antibody (OTI3H3) - Azide and BSA Free (NBP2-01294) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 66.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Ribophorin I Antibody (OTI3H3) - Azide and BSA Free

• DKFZp686B16177
• dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
• Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
• dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• EC 2.4.1.119
• oligosaccharyltransferase 1 homolog
• OST1
• ribophorin IRBPH1
• ribophorin-1
• RPN-I

Background

The Ribophorin I gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-S

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPN1