![Western Blot: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribonuclease Inhibitor (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Western-Blot-NBP2-73896-img0002.jpg "Western Blot: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribonuclease Inhibitor (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI1B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | Novus Biologicals Mouse Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free (NBP2-03254) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNH1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 49.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RNH1 |
![Immunocytochemistry/Immunofluorescence: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ribonuclease Inhibitor.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73896-img0004.jpg "Immunocytochemistry/Immunofluorescence: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ribonuclease Inhibitor.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0010.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![Flow Cytometry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Ribonuclease Inhibitor antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73896-img0003.jpg "Flow Cytometry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Ribonuclease Inhibitor antibody, and then analyzed by flow cytometry.")
![Western Blot: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribonuclease Inhibitor monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Western-Blot-NBP2-73896-img0001.jpg "Western Blot: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribonuclease Inhibitor monoclonal antibody.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0005.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0006.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0007.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0008.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Human lymph node tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0009.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Human lymph node tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribonuclease-Inhibitor-Antibody-OTI1B7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73896-img0011.jpg "Immunohistochemistry: Ribonuclease Inhibitor Antibody (OTI1B7) - Azide and BSA Free [NBP2-73896] - Staining of paraffin-embedded Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.")
![N/A Angiogenin [HRP]](https://images.novusbio.com/images2/Angiogenin_DAN00_ELISA_28.jpg)
![N/A Angiogenin [HRP]](https://images.novusbio.com/images2/DATA_Angiogenin_DAN00_ELISA_571.jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")