Ribonuclease A Antibody

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP2-20160] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.

Western Blot: Ribonuclease A Antibody [NBP2-20160] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ribonuclease A antibody (NBP2-20160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Ribonuclease A antibody detects RNASE1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RNASE1 protein stained by Ribonuclease A antibody (NBP2-20160) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ribonuclease A Antibody Summary

• Description: Novus Biologicals Rabbit Ribonuclease A Antibody (NBP2-20160) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ribonuclease A. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Primate (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNASE1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Rhesus Monkey (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Ribonuclease A Antibody

• EC 3.1.27.5
• HP-RNase
• MGC12408
• RIB1
• RIB-1
• Ribonuclease 1
• Ribonuclease A
• ribonuclease pancreatic
• ribonuclease, RNase A family, 1 (pancreatic)
• RNase A
• RNase UpI-1
• RNS1RNase 1

Background

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RNASE1
• Uniprot:
  • P07998(Human)