Ribonuclease A Antibody

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Western Blot: Ribonuclease A Antibody [NB600-648] - Analysis using Biotin conjugate of NB600-648. Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody ...read more
Western Blot: Ribonuclease A Antibody [NB600-648] - Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Ribonuclease A (Bovine Pancreas) Antibody at 1:1,000 overnight at 4C. Secondary ...read more

Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

Ribonuclease A Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Full length protein (Ribonuclease A from Bovine pancreas).
Localization
Secreted
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas]. Cross-reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNASE1
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Ribonuclease A [Bovine pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:40,000 - 1:180,000 is suggested for this product.
Publications
Read Publication using NB600-648.

Reactivity Notes

Cross-reacts with Cow. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Ribonuclease A Antibody

  • EC 3.1.27.5
  • HP-RNase
  • MGC12408
  • RIB1
  • RIB-1
  • Ribonuclease 1
  • Ribonuclease A
  • ribonuclease pancreatic
  • ribonuclease, RNase A family, 1 (pancreatic)
  • RNase A
  • RNase UpI-1
  • RNS1RNase 1

Background

Ribonuclease A is an endonuclease that catalyzes the cleavage of RNA on the 3' side of pryimidine nucleatides. It acts on single stranded and double stranded RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ribonuclease A Antibody (NB600-648)(1)

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Bioinformatics

Gene Symbol RNASE1