RhoGDI Antibody (2G3) - BSA Free

Western Blot: RhoGDI Antibody (2G3) [NBP2-37472] - Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: RhoGDI Antibody (2G3) [NBP2-37472] - Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: RhoGDI Antibody (2G3) [NBP2-37472] - Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).

Western Blot: RhoGDI Antibody (2G3) [NBP2-37472] - Western blot analysis using ARHGDIA mAb against human ARHGDIA recombinant protein. (Expected MW is 48.7 kDa)

ELISA: RhoGDI Antibody (2G3) [NBP2-37472] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Pm, Pm
• Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: 2G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RhoGDI Antibody (2G3) - BSA Free Summary

• Description: Novus Biologicals Mouse RhoGDI Antibody (2G3) - BSA Free (NBP2-37472) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human RhoGDI (AA: FULL(1-204)) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARHGDIA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RhoGDI Antibody (2G3) - BSA Free

• GDIA1
• MGC117248
• Rho GDI 1
• Rho GDP dissociation inhibitor (GDI) alpha
• rho GDP-dissociation inhibitor 1
• Rho-GDI alpha
• RHOGDI
• RHOGDI-1

Background

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGDIA