ARHGDIG Antibody

Western Blot: ARHGDIG Antibody [NBP1-49854] - Western blot analysis of ARHGDIG in HeLa lysate (35ug protein in RIPA buffer) using NBP1-49854 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ARHGDIG Antibody [NBP1-49854] - 3.75ug/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARHGDIG Antibody Summary

• Immunogen: Peptide with sequence C-EAVPEYRAPGRK corresponding to C-Terminus according to NP_001167.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGDIG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-2ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5-2 ug/ml
• Application Notes: Western blot: Approx 25kDa band observed in lysates of cell line HeLa (calculated MW of 25.1kDa according to NP_001167.2). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Brain (Cortex).
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARHGDIG Antibody

• Rho GDI 3
• Rho GDP dissociation inhibitor (GDI) gamma
• rho GDP-dissociation inhibitor 3
• RhoGDI gamma
• Rho-GDI gamma
• RHOGDI-3

Background

ARHGDIG inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms ofpost-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammaliancells. Stimulates the release of the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARHGDIG Antibody (NBP1-49854)(1)

Publication using NBP1-49854

• Wooten EC, Iyer LK, Montefusco MC et al. Application of gene network analysis techniques identifies AXIN1/PDIA2 and endoglin haplotypes associated with bicuspid aortic valve. PLoS One 2010-01-21 [PMID: 20098615]

Bioinformatics

• Gene Symbol: ARHGDIG
• Entrez:
  • 398(Human)
  • 14570(Mouse)
  • 360500(Rat)
• Uniprot:
  • Q99819(Human)