RhoA Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western Blot: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RhoA Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit RhoA Antibody - BSA Free (NBP3-15485) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoA (NP_001655.1). MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHOA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RhoA Antibody - BSA Free

• h12
• oncogene RHO H12
• ras homolog gene family, member A
• Rho cDNA clone 12
• RHO12
• RhoA
• RHOH12ARH12ARHAAplysia ras-related homolog 12
• small GTP binding protein RhoA
• transforming protein RhoA

Background

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOA