| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27177-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15485-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27831-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90107-50ul | |||
![Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.](http://images.novusbio.com/fullsize/RhoA-Antibody-Western-Blot-NB100-91273-img0034.jpg "Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0024.jpg)
![Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-NB100-91273-img0040.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0023.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0029.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0031.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0032.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0016.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on mouse brain (cryo section) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0035.jpg)
![Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Western-Blot-NBP2-22528-img0001.jpg "Western Blot: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of 25 ug of various whole cell lysates or 0.5 ug of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C (green) in NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/B/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of Rho A/B/C (green) showing staining in the in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunocytochemistry-Immunofluorescence-NBP2-22528-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of showing staining in the cytoplasm of mouse breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis showing staining in the cytoplasm of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunohistochemistry-Paraffin-NBP2-22528-img0007.jpg)
![Immunoprecipitation: RhoA Antibody (1A11-4G10) [NBP2-22528] - Analysis of RhoA/B/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 750 ug of HeLa cell lysate with 2 ug of a RhoA/B/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25 ug of HeLa cell lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rho A/B/C monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-4G10-Immunoprecipitation-NBP2-22528-img0002.jpg)
![Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed by loading 25 ug of various whole cell lysates or 0.5 ug of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a RhoA/C monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Western-Blot-NBP2-22529-img0001.jpg "Western Blot: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed by loading 25 ug of various whole cell lysates or 0.5 ug of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a RhoA/C monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunocytochemistry-Immunofluorescence-NBP2-22529-img0005.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of human prostate carcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunohistochemistry-Paraffin-NBP2-22529-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3%% BSA/TBST for 15 minutes at room temperature. Cells were probed with a RhoA/C monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunocytochemistry-Immunofluorescence-NBP2-22529-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of Rho A/C (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunocytochemistry-Immunofluorescence-NBP2-22529-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm of human hepatocarcinoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunohistochemistry-Paraffin-NBP2-22529-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis showing staining in the cytoplasm and membrane of mouse liver tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunohistochemistry-Paraffin-NBP2-22529-img0007.jpg)
![Immunoprecipitation: RhoA Antibody (1B3-4A10) [NBP2-22529] - Analysis of RhoA/C was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 2ug of a RhoA/C monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B3-4A10-Immunoprecipitation-NBP2-22529-img0002.jpg)
![Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against human RhoA (AA: 1-190) recombinant protein.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-10420249181915.jpg%3Fv=1666706023 "Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against human RhoA (AA: 1-190) recombinant protein.")
![Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916249.jpg%3Fv=1666706023)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Jurkat cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-10420249181921.jpg%3Fv=1666706024)
![Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against HEK293 (1) and RhoA (AA: 1-190)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-10420249141942.jpg%3Fv=1666706023)
![ELISA: RhoA Antibody (7E7H4) [NBP3-27177] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024917035.jpg%3Fv=1666706023)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Hela cells using RhoA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024915828.jpg%3Fv=1666706024)
![Immunohistochemistry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of paraffin-embedded gastric cancer tissues using RhoA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916244.jpg%3Fv=1666706024)
![Immunohistochemistry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of paraffin-embedded endometrial carcinoma tissues using RhoA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024915822.jpg%3Fv=1666706026)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of K562 cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916223.jpg%3Fv=1666706024)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of HepG2 cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024915836.jpg%3Fv=1666706023)
![Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Hela cells using RhoA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27177_mouse-rhoa-mab-7e7h4-bsa-free-1042024916248.jpg%3Fv=1666706023)
![Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04), clone 1B12. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Western-Blot-H00000387-M04-img0009.jpg "Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04), clone 1B12. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Immunocytochemistry-Immunofluorescence-H00000387-M04-img0006.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma tissue.Antibody concentration 5 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Immunohistochemistry-Paraffin-H00000387-M04-img0007.jpg)
![Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in HL-60 (Cat # L014V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-Western-Blot-H00000387-M04-img0008.jpg)
![ELISA: RhoA Antibody (1B12) [H00000387-M04] - Detection limit for recombinant GST tagged RHOA is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1B12-ELISA-H00000387-M04-img0004.jpg)
![Western Blot: RhoA Antibody (1C1) [H00000387-M05] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M05), clone 1C1. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Western-Blot-H00000387-M05-img0009.jpg "Western Blot: RhoA Antibody (1C1) [H00000387-M05] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M05), clone 1C1. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RhoA Antibody (1C1) [H00000387-M05] - Analysis of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human tonsil tissue.Antibody concentration 5 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Immunohistochemistry-Paraffin-H00000387-M05-img0005.jpg)
![Western Blot: RhoA Antibody (1C1) [H00000387-M05] - Analysis of RHOA expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Western-Blot-H00000387-M05-img0008.jpg)
![Proximity Ligation Assay: RhoA Antibody (1C1) [H00000387-M05] - Analysis of protein-protein interactions between MAPK8 and RHOA. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-RHOA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction c](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-Proximity-Ligation-Assay-H00000387-M05-img0006.jpg)
![ELISA: RhoA Antibody (1C1) [H00000387-M05] - Detection limit for recombinant GST tagged RHOA is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1C1-ELISA-H00000387-M05-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15485-img0003.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NBP3-15485-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RhoA-Antibody-Knockout-Validated-NBP3-15485-img0001.jpg)
![Immunohistochemistry-Paraffin: Rho A/B/C Antibody (SR1355) [NBP3-27831] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma](http://images.novusbio.com/fullsize/nbp3-27831_rabbit-rho-a-b-c-mab-sr1355-75202410133492.jpg "Immunohistochemistry-Paraffin: Rho A/B/C Antibody (SR1355) [NBP3-27831] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma")
![Western Blot: Rho A/B/C Antibody (SR1355) [NBP3-27831] - Western blot analysis of (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27831_rabbit-rho-a-b-c-mab-sr1355-7520249391320.jpg)
![Immunocytochemistry/ Immunofluorescence: Rho A/B/C Antibody (SR1355) [NBP3-27831] - Immunofluorescent analysis of Hela cells](http://images.novusbio.com/fullsize/nbp3-27831_rabbit-rho-a-b-c-mab-sr1355-752024120324.jpg)
![Western Blot: RhoA Antibody (1C3) [H00000387-M08] - Analysis of RHOA expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1C3-Western-Blot-H00000387-M08-img0005.jpg "Western Blot: RhoA Antibody (1C3) [H00000387-M08] - Analysis of RHOA expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (1C3) [H00000387-M08] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RhoA-Antibody-1C3-Immunocytochemistry-Immunofluorescence-H00000387-M08-img0003.jpg)
![Sandwich ELISA: RhoA Antibody (1C3) [H00000387-M08] - Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1C3-Sandwich-ELISA-H00000387-M08-img0004.jpg)
![Western Blot: RhoA Antibody (1A11) [H00000387-M03] - Analysis of RHOA expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-Western-Blot-H00000387-M03-img0003.jpg "Western Blot: RhoA Antibody (1A11) [H00000387-M03] - Analysis of RHOA expression in HeLa (Cat # L013V1).")
![ELISA: RhoA Antibody (1A11) [H00000387-M03] - Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RhoA-Antibody-1A11-ELISA-H00000387-M03-img0002.jpg)
![Western Blot: RhoA [p Ser188] Antibody [NBP3-23372] - Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (lanes 1 & 2) or with anti-RhoA (Ser-188) (NBP3-23372; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23372_rabbit-rhoa-p-ser188-pab-1992023141013.jpg "Western Blot: RhoA [p Ser188] Antibody [NBP3-23372] - Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho (lanes 1 & 2) or with anti-RhoA (Ser-188) (NBP3-23372; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).")
![Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.](http://images.novusbio.com/fullsize/RhoA-Antibody-103-Western-Blot-NBP2-90107-img0003.jpg "Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody (103) [NBP2-90107] - Immunofluorescence staining of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/RhoA-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-90107-img0002.jpg)