RGM-B Antibody

RGM-B was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglia) using 1.7 µg/mL Sheep Anti-Mouse RGM-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3597) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

RGM-B Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse RGM-B
  Gly49-Ser414
  Accession # Q7TQ33
• Specificity: Detects mouse RGM-B in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) RGM-A and rmRGM-C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: RGMB
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RGM-B Antibody

• DKFZp434P228
• Dragon
• DRG11-responsive axonal guidance and outgrowth of neurite
• RGM domain family, member B
• RGMB
• RGM-B

Background

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and rhesus macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3-5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2-5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

1. Monnier, P.P. et al. (2002) Nature 419:392.
2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGM-B Antibody (AF3597)(1)

Publication using AF3597

• Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 2014;211(5):943-59. 2014 [PMID: 24752301] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: RGMB
• Uniprot:
  • Q7TQ33()