RFX6 Antibody

RFX6 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human RFX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7780) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

RFX6 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human RFX6
  Lys324-Thr511
  Accession # Q8HWS3
• Specificity: Detects human RFX6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: RFX6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RFX6 Antibody

• dJ955L16.1
• DNA-binding protein RFX6
• MGC33442
• MTFS
• Regulatory factor X 6
• Regulatory factor X domain-containing protein 1
• regulatory factor X, 6
• RFX6
• RFXDC1
• RFXDC1regulatory factor X domain containing 1

Background

RFX6 (regulatory factor X, member 6) is a 928 amino acid, ~102 kDa (calculated) member of the RFX family of transcription factors that are critical for development. RFX6 is expressed in the gut endoderm, later becoming restricted to developing and adult pancreatic islets. It is downstream of Neurogenin-3 and essential for differentiation of most islet cell types. Mutation in humans can produce autosomal recessive neonatal diabetes. Human RFX6 shares 96% and 95% amino acid sequence identity with mouse and rat RFX6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFX6 Antibody (AF7780)(1)

Publication using AF7780

• SE Vazquez, EM Ferré, DW Scheel, S Sunshine, B Miao, C Mandel-Bre, Z Quandt, AY Chan, M Cheng, M German, M Lionakis, JL DeRisi, MS Anderson Identification of novel, clinically correlated autoantigens in the monogenic autoimmune syndrome APS1 by proteome-wide PhIP-Seq Elife, 2020-05-15;9(0):. 2020-05-15 [PMID: 32410729] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: RFX6
• Uniprot:
  • Q8HWS3()