GPRC6A Antibody - BSA Free

Immunohistochemistry-Paraffin: GPRC6A Antibody [NLS2576] - Brain, Glioblastoma

Immunohistochemistry-Paraffin: GPRC6A Antibody [NLS2576] - Analysis of anti-GPRC6A antibody with human brain, neurons and glia.

Immunohistochemistry-Paraffin: GPRC6A Antibody [NLS2576] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPRC6A Antibody [NLS2576] - Breast, carcinoma

Immunohistochemistry-Paraffin: GPRC6A Antibody [NLS2576] - Brain, Hypothalamus, neurons of the tuberal nucleus

• Reactivity: Hu, Rt, Pm, Ca, Eq, Pm, Rb
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPRC6A Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 17 amino acid peptide from N-terminal extracellular domain of human GPRC6A.
• Epitope: N-Terminus extracellular domain
• Localization: Cell Membrane
• Specificity: Human GPRC6A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NBPF3 (59%).
• Predicted Species: Equine (94%), Primate (100%), Canine (94%), Rabbit (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPRC6A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 11 ug/ml
• Control:

  GPRC6A Overexpression Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 18221783). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (82%), Mouse (82%), Rat (82%), Bovine (82%), Hamster (82%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPRC6A Antibody - BSA Free

• bA86F4.3
• G protein-coupled receptor, family C, group 6, member A
• GPCR
• GPCR33
• GPRC6A
• G-protein coupled receptor family C group 6 member A
• G-protein coupled receptor GPCR33
• hGPCR33
• hGPRC6A
• predicted with SOSUI analysis
• seven transmembrane helix receptor

Background

GPRC6A has been shown to bind to L-alpha-amino acids

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPRC6A Antibody (NLS2576)(3)

Publications using NLS2576

• Coskun G, Sencar L, Tuli A et al. Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation International Journal of Endocrinology 2019-08-28 [PMID: 31558901] (IF/IHC)
• Toka HR, Al-Romaih K, Koshy JM et al. Deficiency of the calcium-sensing receptor in the kidney causes parathyroid hormone-independent hypocalciuria. J Am Soc Nephrol. 2012-11-01 [PMID: 22997254]
• Harno E, Edwards G, Geraghty AR et al. Evidence for the presence of GPRC6A receptors in rat mesenteric arteries. Cell Calcium. 2008-08-01 [PMID: 18221783] (IHC-P, Rat)
  
  Details:
  IHC [Paraffin] (rat mesenteric arteries), Figs. 1C (right panel), 5A,C.

Bioinformatics

• Gene Symbol: GPRC6A
• Entrez:
  • 222545(Human)
• Uniprot:
  • Q5T6X5(Human)