RFX6 Antibody (811704)

Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RFX6 Monoclonal Antibody (Catalog # MAB7780) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RFX6 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 811704
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

RFX6 Antibody (811704) Summary

• Immunogen: E. coli-derived recombinant human RFX6
  Lys324-Thr511
  Accession # Q8HWS3
• Specificity: Detects human RFX6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RFX6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RFX6 Antibody (811704)

• dJ955L16.1
• DNA-binding protein RFX6
• MGC33442
• MTFS
• Regulatory factor X 6
• Regulatory factor X domain-containing protein 1
• regulatory factor X, 6
• RFX6
• RFXDC1
• RFXDC1regulatory factor X domain containing 1

Background

RFX6 (regulatory factor X, member 6) is a 928 amino acid, ~102 kDa (calculated) member of the RFX family of transcription factors that are critical for development. RFX6 is expressed in the gut endoderm, later becoming restricted to developing and adult pancreatic islets. It is downstream of Neurogenin-3 and essential for differentiation of most islet cell types. Mutation in humans can produce autosomal recessive neonatal diabetes. Human RFX6 shares 96% and 95% amino acid sequence identity with mouse and rat RFX6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFX6 Antibody (MAB7780)(1)

Publication using MAB7780

• Carly M. Darden, Srividya Vasu, Jordan Mattke, Yang Liu, Christopher J. Rhodes, Bashoo Naziruddin et al. Calcineurin/NFATc2 and PI3K/AKT signaling maintains beta -cell identity and function during metabolic and inflammatory stress iScience 2022-04-15 [PMID: 35402865]

Bioinformatics

• Gene Symbol: RFX6
• Uniprot:
  • Q8HWS3()