RFX5 Antibody - BSA Free

Western Blot: RFX5 Antibody [NB100-195] - Nuclear extract (50 ug for WB, 1 mg for IP) from HeLa cells. Antibody used at 1 ug/ml for WB and 4 ug for IP.

Western Blot: RFX5 Antibody [NB100-195] - HeLa Cell Nuclear Extract

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RFX5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RFX5 Antibody - BSA Free (NB100-195) is a polyclonal antibody validated for use in WB and IP. Anti-RFX5 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Rabbits were immunized with a synthetic peptide, which represented a portion of human Regulatory Factor X 5 encoded within exon 5 (LocusLink ID 5993), conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were affinity purified using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1 - 4 ug/mg of lysate
  • Western Blot 1:500-1:5000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RFX5 Antibody - BSA Free

• DNA-binding protein RFX5
• Regulatory factor X 5
• regulatory factor X, 5 (influences HLA class II expression)

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RFX5 Antibody (NB100-195)(3)

Publications using NB100-195

• Yoshimura Y Increased error-free DNA repair gene expression through reprogramming in human iPS cells Regenerative Therapy 2019-06-28 [PMID: 31304203] (WB, Human)
• Richter Katharina N, Wildhagen Hanna, Helm Martin S et al. Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins. Scientific Reports 2018-10-04 [PMID: 30287847]
• Ulbricht T, Alzrigat M, Horch A et al. PML promotes MHC class II gene expression by stabilizing the class II transactivator J Cell Biol 2012-09-24 [PMID: 23007646] (WB, Human)

Bioinformatics

• Gene Symbol: RFX5
• Entrez:
  • 5993(Human)