Rabbits were immunized with a synthetic peptide, which represented a portion of human Regulatory Factor X 5 encoded within exon 5 (LocusLink ID 5993), conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were affinity purified using the peptide immobilized on solid support.
Immunogen affinity purified
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Suggested working dilutions: * Western Blot 1:500 to 1:5,000 Immunoprecipitation 1 to 4 ul Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application. This antibody can be used for Western Blot and Immunoprecipitation.
regulatory factor X, 5 (influences HLA class II expression)
RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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