RFX5 Antibody

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Western Blot: RFX5 Antibody [NB100-195] - Nuclear extract (50 ug for WB, 1 mg for IP) from HeLa cells. Antibody used at 1 ug/ml for WB and 4 ug for IP.
Western Blot: RFX5 Antibody [NB100-195] - HeLa Cell Nuclear Extract

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

RFX5 Antibody Summary

Immunogen
Rabbits were immunized with a synthetic peptide, which represented a portion of human Regulatory Factor X 5 encoded within exon 5 (LocusLink ID 5993), conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were affinity purified using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RFX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1 - 4 ug/mg of lysate
Application Notes
Suggested working dilutions: * Western Blot 1:500 to 1:5,000 Immunoprecipitation 1 to 4 ul Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application. This antibody can be used for Western Blot and Immunoprecipitation.
Control
RFX5 Lysate (NBP2-65586)
Publications
Read Publication using
NB100-195 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RFX5 Antibody

  • DNA-binding protein RFX5
  • Regulatory factor X 5
  • regulatory factor X, 5 (influences HLA class II expression)

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RFX5 Antibody (NB100-195)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RFX5
Entrez