![Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFWD3-Antibody-Western-Blot-NB100-68208-img0004.jpg "Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, Po, Pm, PmSpecies Glossary |
| Applications | WB, IP (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | Novus Biologicals Rabbit RFWD3 Antibody (NB100-68208) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 724 and 774 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159). |
| Predicted Species | Porcine (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RFWD3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunoprecipitation: Not recommended. |
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| Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry/ Immunofluorescence RPA2 [p Ser4, p Ser8] Antibody - BSA Free](https://images.novusbio.com/images/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-23017-img0046.jpg)
![Western Blot RPA2 [p Ser4, p Ser8] Antibody - BSA Free](https://images.novusbio.com/images/RPA2-[p-Ser4--p-Ser8]-Antibody-Western-Blot-NBP1-23017-img0047.jpg)
![Immunoprecipitation RPA2 [p Ser4, p Ser8] Antibody - BSA Free](https://images.novusbio.com/images/RPA2-[p-Ser4--p-Ser8]-Antibody-Immunoprecipitation-NBP1-23017-img0048.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for RFWD3 Antibody (NB100-68208)Find related products by research area.
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| Gene Symbol | RFWD3 |
| Entrez |
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| Uniprot |
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![Immunoprecipitation: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFWD3-Antibody-Immunoprecipitation-NB100-68208-img0005.jpg "Immunoprecipitation: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")