RFWD3 Antibody

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Immunoprecipitation: RFWD3 Antibody [NB100-68206] - Detection of Human RFWD3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

RFWD3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RFWD3
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 5 ug/mg lysate
Application Notes
This antibody is not recommended for Western Blot analysis.
Positive Control
RFWD3 Lysate (NBL1-15303)
Reviewed Applications
Read 1 Review rated 4
using
NB100-68206 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RFWD3 Antibody

  • EC 6.3.2.-
  • FLJ10520
  • ring finger and WD repeat domain 3
  • RING finger and WD repeat domain-containing protein 3
  • RING finger protein 201
  • RNF201E3 ubiquitin-protein ligase RFWD3

Background

RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RFWD3 Antibody (NB100-68206) (0)

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Review for RFWD3 Antibody (NB100-68206) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-68206:
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WB
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Kerstin Knies
WB Human 02/28/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate of human fibroblasts
SpeciesHuman

Blocking

Blocking DetailsBlocking milk 5% in PBS-T over night at 4°C

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti rabbit IgG HRP
Secondary Concentration1:10000

Details

Detection NotesHRP Substratet of Millipore, exposure time 2 min.

Product General Protocols

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FAQs for RFWD3 Antibody (NB100-68206) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RFWD3 Antibody (NB100-68206)

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Kerstin Knies
02/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RFWD3
Entrez
Uniprot