RFNG Antibody - BSA Free

Western Blot: RFNG Antibody [NBP1-90946] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunocytochemistry/Immunofluorescence: RFNG Antibody [NBP1-90946] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: RFNG Antibody [NBP1-90946] - Staining of human kidney shows cytoplasmic, nuclaer and membranous positivity.

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RFNG Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RFNG Antibody - BSA Free (NBP1-90946) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AGFCLSRGLALKMSPWASLGSFMSTAEQVRLPDDCTVGYIVEGLLGARLLHSPLFHSHLENLQRLPPDTLLQQVTLSHGGPENPHNVVNVAGGFSLHQDPTRFKSIHCLLYPDTDWCPRQKQGA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFNG
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RFNG Protein (NBP1-90946PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RFNG Antibody - BSA Free

• beta-1,3-N-acetylglucosaminyltransferase radical fringe
• EC 2.4.1.222
• O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
• radical fringe (Drosophila) homolog
• radical fringe homolog (Drosophila)
• radical fringe homolog
• RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
• RFNG

Background

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFNG