Rffl Antibody - BSA Free

Western Blot: Rffl Antibody - BSA Free [NBP3-46753] - Mouse testis tissue were subjected to SDS PAGE followed by western blot with (Rffl antibody) at dilution of 1:500

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Rffl Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Rffl Antibody - BSA Free (NBP3-46753) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ring finger and FYVE-like domain containing 1. (Uniprot# Q8WZ73)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RFFL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 37-41 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Rffl Antibody - BSA Free

• CARP-2
• Caspase regulator CARP2
• Caspases-8 and -10-associated RING finger protein 2
• E3 ubiquitin-protein ligase rififylin
• EC 6.3.2
• EC 6.3.2.-
• Fring
• FYVE-RING finger protein Sakura
• rififylin
• ring finger and FYVE-like domain containing 1
• RING finger and FYVE-like domain-containing protein 1
• RING finger protein 189
• RING finger protein 34-like
• RNF189CARP2
• RNF34LFRING

Background

RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFFL