RFC4 Antibody (OTI1A8) - Azide and BSA Free

Images

 
Western Blot: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.
Immunocytochemistry/ Immunofluorescence: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.
Immunohistochemistry: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of extracts (35ug) from 9 different cell lines by using RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; ...read more
Immunohistochemistry: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: RFC4 Antibody (OTI1A8) - Azide and BSA Free [NBP2-73859] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

RFC4 Antibody (OTI1A8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RFC4(NP_002907) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RFC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:200-4000
Theoretical MW
39.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for RFC4 Antibody (OTI1A8) - Azide and BSA Free

  • A1 37 kDa subunit
  • A1
  • Activator 1 37 kDa subunit
  • Activator 1 subunit 4
  • MGC27291
  • replication factor C (activator 1) 4, 37kDa
  • Replication factor C 37 kDa subunit
  • replication factor C subunit 4
  • RFC 37 kDa subunit
  • RF-C 37 kDa subunit
  • RFC37replication factor C (activator 1) 4 (37kD)

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RFC4