Resistin Antibody [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

Resistin Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse Resistin (R&D Systems, Catalog # 1069-RN)
  Ser21-Ser114
  Accession # Q99P87
• Specificity: Detects mouse Resistin in direct ELISAs and Western blots. In this format, less than 2% cross-reactivity with recombinant human Resistin and recombinant mouse RELM beta is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Resistin Antibody [Alexa Fluor® 532]

• Adipose tissue-specific secretory factor
• ADSF
• ADSFMGC126609
• C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1
• C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
• Cysteine-rich secreted protein A12-alpha-like 2
• Cysteine-rich secreted protein FIZZ3
• FIZZ3
• FIZZ3MGC126603
• found in inflammatory zone 3
• HXCP1
• Resistin
• RETN
• RETN1
• RSTNXCP1

Background

Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance. Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha -helical domain and a C-terminal beta -sandwich domain that is stabilized by multiple intrachain disulfide bonds. Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity. Resistin can also form multimers with RELM beta . Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha , beta , and gamma . In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus. It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet. This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes. Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance. Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species. Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.